Rotor Gene 6000 Real-Time PCR Machine Features: • Unique centrifugal rotary format • Uniformity: ± 0.01°C • Resolution: 0.02°C (smallest programmable and reported thermal increment) • 5-Plex (Green, Yellow, Orange, Red, Crimson) fluorescence channels • High resolution melt (HRM) application • Holds 36 x0.2ml tubes, 72 x 0.1ml strip tubes or 100 Gene-Disc • Software: Build-in extensive analysis, graphing and statistical functions. Unlimited use software license for Windows XP, Pentium IV (2GHz) or higher PC. Rotor Gene 3000 Real-Time PCR Machine Features: • Unique centrifugal rotary format • Excitation Source: LED high power diodes, 470nm, 530nm, 585nm and 625nm • Detection Filters: 510nm (FAM), 555nm (JOE), 610nm (ROX) and 660nm high-pass (Cy5) • Holds 36 x0.2ml tubes or 72 x 0.1ml strip tubes • Software: Build-in extensive analysis, graphing and statistical functions. Use of the machines in the Molecular Biology Facility • The Rotor-Gene machines are in high demand therefore there is an (external website). Plan ahead, book time slots and stick to them. • Users are responsible for supplying all their own plastic ware and reagents.

Set-up, programming and analysis: RotorGene 3000/6000, Rotor-Gene Q. Start the Rotor-Gene software. For use on the Rotor-Gene Q. Rotor-Gene Q Software 2.3 is compatible with both Windows XP and Windows 7 operating systems. Download Contact QIAGEN.

0.1ml tubes and Gene-Disc 100 are produced by Corbett especially for the Rotor-Gene. • Save files in your own folder in the 'Rotor Gene Run File' folder in the local disk (C:). The computer is also linked to the network for easy transfer of your data to the server.

Tips and addition information • The Rotor-Gene machines support reaction volumes from 5 µl to 100 µl (rotor and protocol dependent). Typical reaction volume is 20µl.

• The use of a normalisation dye, such as ROX reference dye, is optional on the Rotor-Gene. • Many companies supply master mixes for real-time PCR which can decrease the chance of pipetting errors in this highly sensitive method. • If you would like to learn more about the real-time PCR experimental design and data analysis, please contact Dr.

Mibac Music Lessons I: Fundamentals Free Download Programs here. Donna Lai, the Molecular Biology Officer. Instructional Rating Manual Skydiving Chicago. Bioanalyzer, Real-time RT-PCR workshops are conducted occasionally during the year. Please contact Dr.